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. 2000 Aug;11(8):2565–2575. doi: 10.1091/mbc.11.8.2565

Figure 7.

Figure 7

Effects of dominant negative mutants of Vav2 on the stress fiber formation in MDCK-SHP-2-C/S cells and wild-type MDCK cells. MDCK-SHP-2-C/S cells were transfected with pCIneo-myc-Vav2-ΔDH+PH (ΔDH+PH) (Aa and Ab), pCIneo-myc-Vav2-L212Q (L212Q) (Ac and Ad), pCIneo-myc-Vav2-ΔDH+PH/R688K (ΔDH+PH/R688K) (Ba and Bb), pCIneo-myc-Vav2-L212Q/R688K (L212Q/R688K) (Bc and Bd), or pCIneo-myc-Vav2-wild-type (WT) (Ca and Cb). Wild-type MDCK cells were also transfected with pCIneo-myc-Vav2-ΔDH+PH (ΔDH+PH) (Cc and Cd). At 48 h after transfection, the cells were fixed, double stained with rhodamine-conjugated phalloidin (Actin) or the anti-Myc mAb (Myc), and analyzed by confocal microscopy. Confocal images are shown at the basal level. The results shown are representative of three independent experiments. Bar, 10 μm.