Figure 8.

Inhibitory effect of Y172F or the ΔN mutant of Vav2 on HGF/SF-induced cell scattering of MDCK cells. Wild-type MDCK cells were microinjected with pCIneo-myc-Vav2-Y172F (Y172F) (Aa–Ad) or pCIneo-myc-Vav2-ΔN-terminal region (ΔN) (Ba–Bd). At 24 h after microinjection, the cells were stimulated with no HGF/SF (Aa, Ac, Ba, and Bc) or 10 ng/ml HGF/SF (Ab, Ad, Bb, and Bd). At 18 h after HGF/SF stimulation, the cells were double stained with rhodamine-conjugated phalloidin (Actin; Aa, Ab, Ba, and Bb) or the anti-Myc mAb (Ac, Ad, Bc, and Bd) and analyzed by confocal microscopy. Confocal images are shown at the basal level. The results shown are representative of three independent experiments. Bars, 10 μm.