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H89 blocks hypotonically induced GPP130 tubules. HeLa cells grown to 50% confluence on 12-mm glass coverslips were placed in 1 ml of normal medium (A), normal medium containing 50 μM H89 (B), hypotonic (210 mOsm) medium (C), or hypotonic medium containing 50 μM H89 (D) for 10 min at 37°C.
