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H89 blocks both BFA- and nocodazole-induced redistribution of GPP130. HeLa cells grown to 50% confluence on 12-mm glass coverslips were incubated in 2.5 μg/ml BFA in the absence (A) or presence (B) of 50 μM H89 for 20 min at 37°C, or incubated in 10 μg/ml nocodazole in the absence (C) or presence (D) of 50 μM H89 for 60 min at 37°C.
