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H89 dose response for inhibition of hypotonically (A), BFA (B)-, and nocodazole (C)-induced redistribution. Cells were treated with hypotonic medium (210 mOsm), 2.5 μg/ml BFA, or 10 μg/ml nocodazole in the presence of 0, 25, 50, and 100 μM H89 or 120 μM H8. Hypotonically treated cells were stained with GM130 antibodies and BFA- and nocodazole-treated cells were stained with GPP130 antibodies. The averaged results of two independent experiments are presented.
