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H89 does not block hypotonically induced ERGIC 53 redistribution to the ER, and H89 alone induces the redistribution of ERGIC 53 to the ER. HeLa cells grown to 50% confluence on 12-mm glass coverslips were placed in 1 ml of normal medium (A), hypotonic (210 mOsm) medium (B), hypotonic medium containing 50 μM H89 (C), or normal medium containing 50 μm H89 (D) at 37°C for 20 min.
