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H89 treatment induces, and does not block the constitutive recycling of GM130 to the ER (A and B), but does block the nocodazole-induced redistribution of GM130 (C and D). HeLa cells grown to 50% confluence on 12-mm glass coverslips were incubated in the absence (A) or presence (B) of 50 μm H89 for 120 min. In C and D, cells were incubated in the presence of 10 μg/ml nocodazole for 60 min (C), or in the presence of 50 μM H89 and 10 μg/ml nocodazole for 60 min (D).
