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Interaction of scRan with Ntf2p. Interactions were examined using bead-binding assays as described in Materials and Methods. Immunoblots detecting scRan are shown. (A) Interaction of scRan with Ntf2–1p and Ntf2–2p. Lane 1, yeast cell lysate; lane 2, wild-type Ntf2p beads; lane3, Ntf2–1p beads; and lane 4, Ntf2–2p beads. (B) Interaction of scRan-GFP with Ntf2p. Lane 1, yeast cell lysate; lane 2, BSA beads; and lane 3, Ntf2p beads.
