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. 2000 Aug;11(8):2617–2629. doi: 10.1091/mbc.11.8.2617

Figure 5.

Figure 5

Figure 5

The ntf2–2ts MAD2Δ double mutant is hypersensitive to microtubule-destabilizing drugs. (A) ntf2–2ts is a unique benomyl-sensitive allele of NTF2. NTF2, ntf2–2ts, ntf2–1ts, ntf2–2ts MAD2Δ, and ntf2–1ts MAD2Δ cells were grown at 25°C overnight, and 100,000, 10,000, 1000, 100, and 10 cells were spotted onto YEPD (left) and 10 μg/ml benomyl plates (right). Plates were incubated at 25°C for 4 days. (B) The ntf2–2ts MAD2Δ double mutant is hypersensitive to nocodazole. NTF2, MAD2Δ, ntf2–2ts, and ntf2–2ts MAD2Δ were grown at 25°C overnight, were diluted to 0.2 × 106 cells/ml into YEPD containing 15 μg/ml nocodazole, and growth was monitored by OD600.