Figure 3.

Introduction of the wild-type TSA1 gene into a tsa1Δ strain restores the normal H₂O₂ induction of TRX2, TRR1, and CTT1. Northern blot analysis of RNA isolated from a midlog-phase culture of a tsa1Δ strain containing either Ycplac111 (lanes 1 and 2) or WT.TSA1 (lanes 3 and 4), which were either untreated (lanes 1 and 3) or treated with 0.1 mM H₂O₂ for 20 min (lanes 2 and 4) with the use of probes specific for TRX2 (A), TRR1 (B), or CTT1 (C) and ACT1. The panels below the Northern blots show quantitation of the different transcripts relative to the ACT1 transcript.