Figure 5.

Northern blot analysis of RNA isolated from different midlog-phase cultures either untreated (lanes 1, 3, 5, 7, 9, and 11) or treated with 0.1 mM H₂O₂ (lanes 2, 4, 6, 8, 10, and 12) for 20 (A) and 40 (B) min with the use of probes specific for the TRX2 and ACT1 transcripts. The strains were W303-1a (lanes 1 and 2), tsa1Δ (lanes 3 and 4), skn7Δ (lanes 5 and 6), tsa1Δskn7Δ (lanes 7 and 8), yap1Δ (lanes 9 and 10), and tsa1Δyap1Δ (lanes 11 and 12). The panels below the Northern blots show quantitation of the different transcripts relative to the ACT1 transcript. (C) Kinetics of TRX2 induction. This graph shows the induction of TRX2 expression in the W303-1a, tsa1Δ, skn7Δ, tsa1Δskn7Δ, yap1Δ, and tsa1Δyap1Δ strains during 40 min of incubation with 0.1 mM H₂O₂.