Figure 6.

Overexpression of TSA1. (A) Spot tests showing the sensitivity to tBOOH after overexpression of TSA1. Strains were W303-1a, skn7Δ, and yap1Δ containing either the Yep24 vector or the Yep24-TSA1 plasmid. (B) Northern blot analysis of RNA isolated from different midlog-phase cultures either untreated (lanes 1, 3, 5, 7, 9, and 11) or treated with 0.1 mM H₂O₂ for 20 min (lanes 2, 4, 6, 8, 10, and 12) with the use of probes specific for TRX2 and ACT1. Strains were W303-1a containing Yep24 (lanes 1 and 2), W303-1a containing Yep24-TSA1 (lanes 3 and 4), yap1Δ containing Yep24 (lanes 5 and 6), yap1Δ containing Yep24-TSA1 (lanes 7 and 8), skn7Δ containing Yep24 (lanes 9 and 10), and skn7Δ containing Yep24-TSA1 (lanes 11 and 12). The panel below the Northern blots shows quantitation of the TRX2 transcript relative to the ACT1 transcript.