FIG. 3.

AhR-mediated ERα degradation occurs in situ. (A) ZR-75 cells were treated with DMSO (D), 10 nM E, 10 nM TCDD (T), or ET for 24 h, and ERα was immunostained as described in Materials and Methods. Field width is 150 μm. (B) Immature (25-day-old) B6C3F1 mice were injected intraperitoneally with corn oil (C), 200 ng of E, or 1 μg of TCDD (T) or ET for 12 h. Uteri were fixed and immunostained as described in Materials and Methods. Field width is 240 μm. Control fields represent background without ERα H184 antibody.