Skip to main content
. 2003 Mar;23(6):1843–1855. doi: 10.1128/MCB.23.6.1843-1855.2003

FIG. 7.

FIG. 7.

Proteasome-dependent degradation of ERα; role of mRNA levels, protein synthesis, and kinase inhibitors. (A) mRNA levels. T47D cells were treated for 6 h with DMSO (D) or TCDD (T) following a 30-min pretreatment with DMSO, 10 μM MG132, or 10 μM PSI, and ERα, AhR, Arnt, and β-tubulin mRNA levels were determined as described in Materials and Methods. (B) Effects of cycloheximide. ZR-75 cells were treated for 3 h with DMSO (D) or 10 nM TCDD (T) following a 30-min pretreatment with MeOH or 25 μM cycloheximide, and whole-cell lysates were analyzed for AhR, ERα, and CYP1A1 proteins by Western blot analysis as described in Materials and Methods. ns, nonspecific protein. (C) Effects of kinase inhibitors. ZR-75 cells were treated with DMSO (D), 10 nM E, 10 nM TCDD (T), and ET for 3 h following a 30-min pretreatment with 10 μM PP2, 1 μM bis-indolylmaleimide 1, 1 μM KT5720, 2 μM manumycin A, 5 μM U0126, or 400 nM wortmannin. ERα and Sp1 proteins were determined by Western blot analysis as described in Materials and Methods.