FIG. 7.

Preferential K88E repression of PITX2a activation of the tandem bicoid element. CHO cells were transfected by lipofection with 1 μg of Bcd 1x (circles) or Bcd 2 × 5n (squares) reporter plasmids and increasing amounts of WT PITX2a (0.01, 0.05, 0.1, 0.25, and 0.5 μg, as indicated) in the absence (solid line) or presence (dotted line) of equal amounts of K88E. The activity was normalized to that of Bcd 2 × 5n with 0.5 μg of WT PITX2a, which was set at 100%. The means ± standard errors of the means from three to four separate experiments are shown. WT PITX2a shows significantly higher activation of the Bcd 2 × 5n reporter than the Bcd 1x reporter (*, P < 0.04; **, P < 0.01; ***, P < 0.005).