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. 2003 Mar;23(6):1994–2008. doi: 10.1128/MCB.23.6.1994-2008.2003

FIG. 6.

FIG. 6.

Direct interaction of PRB and the c-Src SH3 domain. (A) In vitro-translated 35S-labeled PRs (wild type and mutants) were incubated with GST or GST-SrcSH3 fusion protein immobilized on glutathione-Sepharose. Bound proteins were analyzed by SDS-PAGE and fluorography. (B) Yeast two-hybrid assays with wild-type or mutant PRB and ERα. The yeast strain AH109 was cotransformed with Gal4DBD-ERα plus the indicated fusions of Gal4AD with PR (wild type and mutants). Selection was performed as described in the legend to Fig. 2B.