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. 2003 Mar;23(6):1994–2008. doi: 10.1128/MCB.23.6.1994-2008.2003

FIG. 9.

FIG. 9.

Transactivation of an MMTV-Luc reporter gene by progestins in COS-7 cells. COS-7 cells were transiently cotransfected with ERα and PR (PRB wild type, PR ΔERID-I, PR ΔERID-II, or PR ΔERID I+II) expression vectors together with a reporter gene containing the MMTV promoter linked to the luciferase gene (37). Cells were incubated with (shaded bars) or without (black bars) 10 nM R5020 for 24 h, and the luciferase activity was determined in whole-cell extracts (37). The average and standard deviation of two experiments performed in duplicate are shown.