Table 3.

Comparison of the experimental and calculated recycling efficiencies of TR mutants

Protein	Recycling efficiency (%)	Experimental half-life (h)	Calculated half-life (h)
Wild-type TR	98a	27  ± 4.6b (6)c	23.9
TRTM Thr11Gln17Thr19	93	5.4  ± 0.8 (7)	7.4
IiTM 11–19	92	5.6  ± 0.7 (7)	6.5
IiTM 11–19 Ala11Ala17Ala19	96	15  ± 1.9 (7)	12.3

^a Based on 2% TCA-soluble counts in the media after 2 h. See MATERIALS AND METHODS for details of the calculation. 

^b Mean ± SE. 

^c Number of independent experiments.