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HGK kinase activity is required for growth of RIE-1 cells in soft agar but not growth in monolayer. (A) Shown are photomicrographs (10× magnification) of colonies formed in soft agar from RIE-1 stable clones after 28 days of growth. (B) Shown is a semilogarithmic plot of growth curves generated for the same six RIE clones shown in A over a 4-day period, with duplicate wells counted every 24 h.
