FIG. 8.

HGK kinase activity interferes with cell attachment. RIE-1 stable clones expressing active and inactive alleles of HGK as well as vector controls were detached with trypsin and plated on various concentrations of fibronectin (FN). After 60 min, unattached cells were washed away, and the remaining cells were stained with crystal violet. (A) Photomicrographs (2× magnification) show the presence of remaining cells at different concentrations of fibronectin. (B) By comparison with a standard curve of cells plated on polylysine, the relative amount of cells was quantified by measuring the amount of solubilized dye by optical density. The graph shows the relative optical density at 550 nm as a function of fibronectin concentration for the different RIE-1 clones.