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. 2003 Mar;23(6):2171–2181. doi: 10.1128/MCB.23.6.2171-2181.2003

FIG. 2.

FIG. 2.

CP-31398 does not phosphorylate p53 at ser15 or ser20. (A) Adriamycin (ADR) or CP-31398 was added to H460 cell culture at various concentrations and incubated for 4 h. Cell lysates were subjected to SDS-PAGE and probed with anti-p53 (Ab-2) and anti-phospho-p53 antibodies against ser15, ser20, ser46, and ser392, respectively. (B) H460 cells were treated with ADR at a concentration of 0.2 μg/ml or CP-31398 at a concentration of 15 μg/ml. Cells were lysed at different time points after treatment and subjected to SDS-PAGE and immunoblotting with anti-phospho-ser20 of p53.