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. 2003 Mar;23(6):2171–2181. doi: 10.1128/MCB.23.6.2171-2181.2003

FIG. 3.

FIG. 3.

CP-31398 does not prevent the binding of MDM2 to p53. (A) H460 cells were treated with adriamycin (ADR) or CP-31398 (CP) for 4 h. p53 was immunoprecipitated (IP) with anti-p53 (FL-393; Santa Cruz Biotech). The immunoprecipitated complexes were subjected to SDS-PAGE and probed with anti-p53 (Ab-2; Calbiochem) and anti-MDM2 (Ab-1; Calbiochem) antibodies. (B) H460 cells were infected with Ad/His-p53 and treated with adriamycin or CP-31398. p53 was precipitated with Ni-nitrilotriacetic acid resin prior to SDS-PAGE. MDM2 and p53 were probed with the same antibodies as in panel A. WB, Western blotting; C, control.