Figure 8.

EEA1 and expressed endotubin label distinct endosomal populations in NRK cells. NRK cells were transfected with endotubin and after 24 h were labeled for EEA1 (A and D) and endotubin (B and E). The two markers label distinct endosomal elements, as shown in the overlays (C and F). This is particularly evident in peripheral areas of the cell (C, arrows), which contain endotubin-positive elements and no EEA1. The EEA1 and endotubin staining in the central area of the transfected cell in panels A–C is shown in panels D and E, respectively. Note the general lack of colocalization despite the close proximity of labeled elements. Bars, 10 μ m.