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spt mutations allow HO expression in the absence of Gcn5. S1 nuclease protection assays were performed with probes specific for HO and CMD1 (internal control). RNAs were prepared from the following strains: (A) DY150, DY5925, DY1696, and DY5929; (B) DY150, DY5925, DY6219, and DY6277; (C) DY150, DY5925, DY6178, and DY6422; (D) DY151, DY5926, and DY6603; (E) FY61 and FY1006; (F) DY150 and DY6707; (G) DY150 and DY6404.
