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. 2003 Mar;23(6):1874–1884. doi: 10.1128/MCB.23.6.1874-1884.2003

FIG. 2.

FIG. 2.

Proteins cross-link specifically to N1. (A) UV cross-linking to BS303 and BS304 site-specifically labeled at position EX3 in HeLa (H) and Weri-1 (W) nuclear extracts. The grey bar in intron B in BS303 and BS304 represents adenovirus acceptor sequence. This sequence has previously been shown to increase overall splicing levels but not to affect neural specificity. The nucleotides in the exon are designated EX1 through EX23. The mutated nucleotides are underlined. The asterisk denotes the site of the radioactive phosphate in the exon N1 sequence. Arrows highlight protein bands enriched in Weri-1 extract in lane 2. (B) UV cross-linking to BS7 labeled at position EX3 in HeLa and Weri-1 nuclear extracts. (C) UV cross-linking to BS7 site-specifically labeled at EX8 in HeLa and Weri-1 nuclear extracts.