Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Rfm1 is not required for silencing at HMR. The wild-type (WT; W303-1A), sir3Δ (JRY3182), and rfm1Δ (RMY25) strains contain TRP1 integrated at HMR. The strains were grown to mid-log phase, normalized for concentration, and serially diluted fivefold, and then equivalent quantities of all of the dilutions were spotted onto YEPD and SD-Trp plates.
