FIG. 3.
C-terminal dimerization is a prerequisite for IKKγ tetramerization. Labeled FLAG-tagged IKKγ mutants were prepared by in vitro translation. After EGS cross-linking, products were precipitated with anti-FLAG antibodies and analyzed by SDS-PAGE and autoradiography. The positions of molecular mass markers are indicated. (A) Analysis of full-length IKKγ and internal deletion mutants IKKγΔ246-302, IKKγΔ302-365, and IKKγΔ246-365. Migration of monomers and tetramers is indicated. (B) (Top) Analysis of N- and C-terminal IKKγ deletion mutants. Major and minor multimeric forms are indicated below the autoradiogram (minor forms shown in parentheses). (Bottom) Schematic presentation of major oligomerization states of IKKγ subregions. The positions of alpha-helical regions (open boxes), coiled-coil domains (black bars), and zinc finger are shown.