Skip to main content
. 2003 Mar;23(6):2109–2122. doi: 10.1128/MCB.23.6.2109-2122.2003

FIG. 5.

FIG. 5.

HEI10 expression limits mitotic entry and limits cyclin B-Clb2p accumulation. (A) Nuclear envelope breakdown of Xenopus extracts after sperm addition in the presence of GST-HEI10 (open bars), buffer (shaded bars), or GST (solid bars). (B) Egg lysates shown after incubation for the times indicated after sperm addition in the presence of GST, GST-HEI10, or GST-HEI10-ΔRING were used for Western analysis with anti-cyclin B (top) or tubulin (bottom). (C) Western analysis of Clb2p-HA levels in yeast transformed with pJG4-4 vector, HEI10, or HEI10-ΔRING. The anti-tubulin control (TUB2) is shown at the bottom.