Table 1.
S. cerevisiae strains used in this study
| Strain | Genotype | Reference or source |
|---|---|---|
| SEY6210 | MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 | Robinson et al. (1988) |
| SEY5188 | MATα sec18-1 ura3-52 leu2-3,112 suc2-Δ9 | Graham and Emr (1991) |
| AAY102 | SEY6210; stt4Δ::HIS3 carrying pRS415stt4-4 (LEU2 CEN6 stt4-4) | This study |
| AAY104 | SEY6210; pik1Δ::HIS3 carrying pRS314pik1-83 (TRP1 CEN6 pik1-83) | This study |
| AAY105 | SEY6210; stt4Δ::HIS3 pik1Δ::HIS3 carrying pRS415stt4-4 | This study |
| (LEU2 CEN6 stt4-4) and pRS314pik1-83 (TRP1 CEN6 pik1-83) | ||
| AAY107 | SEY6210; mss4Δ::HIS3MX6 carrying pYCplac111mss4-2 (LEU2 CEN6 mss4-2) | This study |
| 6210arf1Δ | SEY6210; arf1Δ::HIS3 | Gaynor et al. (1998) |
| AAY114 | SEY6210; stt4Δ::HIS3 arf1Δ::HIS3 carrying pRS415stt4-4 (LEU2 CEN6 stt4-4) | This study |
| AAY116 | SEY6210; pik1Δ::HIS3 arf1Δ::HIS3 carrying pRS314pik1-83 (TRP1 CEN6 pik1-83) | This study |