FIG. 3.

Orc2, Mcm2, and Orc5 transcripts are elevated in E2f2, Dp, and Rbf1 mutant follicle cells. Relative levels of Orc5 (A), Orc2 (B), and Mcm2 (C) transcripts were determined in wild-type and in E2f2³²⁹/E2f2³²⁹, Df(2R)vg56/Dp^a1, and Rbf¹²⁰/Rbf¹⁴ mutant total follicle cell RNA preparations by the TaqMan quantitative RT-PCR method. The PCR product was quantified by determining the fluorescence intensity at one cycle in the linear phase of the amplification. The fluorescence values of Orc5, Orc2, and Mcm2 were compared between different genotypes by normalizing them to the value obtained for Rp49. All experiments were performed in triplicate, except for Orc5 in wild-type, E2f2, and Rbf1 genotypes, which were performed four times. The data are presented as an average of the fold increases relative to the wild type for each of these experimental trials. (D) An unpaired, two-tailed Student t test was used to determine level of significance of changes in expression between different genotypes. P values for each pairwise combination of experiments is shown.