TABLE 1.
Replication genes are derepressed in E2f2 and Rbf1 mutantsa
| Complex or factor | Ratio of mutant/wild-type hybridization signal
|
|
|---|---|---|
| E2f2 mutant | Rbf1 mutant | |
| Replication initiation complex | ||
| Orc1 | 0.9 | 1.3 |
| Orc2 | 1.3 | 1.3 |
| Orc3 (lat) | 0.8 | 0.9 |
| Orc4 | 1.2 | 1.7 |
| Orc5 | 1.2 | 1.3 |
| Orc6∗ | 1.5 | 1.7 |
| Mcm2∗ | 1.6 | 2.1 |
| Mcm3 | 1.3 | 1.6 |
| Mcm4 (dpa) | 1.4 | 2.8 |
| Mcm5∗ | 1.6 | 2.6 |
| Mcm6∗ | 1.5 | 2.4 |
| Mcm7 | 1.3 | 2.5 |
| dup (CBT1) | 1.3 | 2.0 |
| chif (DBF4) | 1.0 | 0.8 |
| CDC45L | 0.9 | 1.2 |
| Replication factors | ||
| awd (NDP kinase) | 0.9 | 0.9 |
| Dhfr | 1.0 | 1.2 |
| deoxynucleoside kinase (dnk) | 1.1 | 2.9 |
| nmdyn-D6 (NDP kinase) | 0.7 | 0.6 |
| nmdyn-D7 (NDP kinase) | 1.5 | 0.8 |
| RnrL | 1.3 | 2.4 |
| RnrS | 1.2 | 2.5 |
| Ts | 1.3 | 1.5 |
| PCNA (mus209) | 1.1 | 2.2 |
| CG10262 (PCNA2) | 0.5 | 0.6 |
| CG8142∗ (RfC) | 1.6 | 1.6 |
| Gnf1 (RfC) | 1.5 | 1.3 |
| RfC3 | 0.7 | 1.0 |
| RfC38 | 1.4 | 1.5 |
| RfC40 | 1.0 | 1.0 |
| DNApol-α180 | 1.2 | 1.4 |
| DNApol-α50∗ | 2.0 | 2.8 |
| DNApol-α73∗ | 1.7 | 1.9 |
| DNApol-δ | 1.3 | 0.8 |
| CG10489 (DNApol-ɛ) | 0.6 | 0.7 |
| DNApol-ɛ∗ | 1.7 | 1.7 |
| DNApol-γ35 | 0.8 | 0.9 |
| DNAprimase | 1.3 | 4.0 |
| RpA-30 | 1.0 | 1.7 |
| RpA-70 | 1.1 | 2.0 |
| Chromatin assembly | ||
| Acf1∗ | 1.6 | 1.5 |
| Caf1-105 | 1.2 | 1.4 |
| Caf1-180∗ | 1.6 | 2.8 |
| Caf1 | 1.2 | 1.4 |
| Iswi | 1.5 | 1.1 |
| Chromatid cohesion | ||
| Cap (SMC3) | 0.8 | 0.9 |
| mei-S332∗ | 1.8 | 2.3 |
| SA | 1.1 | 1.3 |
| SMC1 | 0.9 | 1.2 |
a
The data are presented as a ratio of mutant to wild-type hybridization signals obtained from an average of two independent DNA microarray experiments for each follicle cell genotype. Genes that exhibited a ≥1.5-fold increase in both E2f2 and Rbf1 mutant follicle cells are indicated by an asterisk.