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. 2003 Mar;77(6):3851–3858. doi: 10.1128/JVI.77.6.3851-3858.2003

FIG. 3.

FIG. 3.

Viral DNA amplification after infection of human and mouse cells with chimeric and pseudotyped viruses. (A and B) NB-324K and A9 cells were infected at a MOI of 0.001 RU/cell (A) or 0.03 RU/cell (B) (MVMp-based virus) and 3 RU/cell (B) (hH1-based virus) with the indicated virus stocks and transferred to nitrocellulose membranes 48 h postinfection. Titration of recombinant viruses was performed by hybridization assay and autoradiography (16, 17), using a NS-specific DNA probe as described in the legend to Fig. 2. (C) Southern blotting analysis of low-molecular-weight DNA, isolated by the modified Hirt procedure, 72 h after infection of A9 cells with the indicated virus stocks at a MOI of 3 RU/cell. DNA samples were fractionated by agarose gel electrophoresis, transferred to Hybond N+ nylon membrane, and hybridized with a NS-specific DNA probe. The positions of monomer- and dimer-length RFs are indicated.