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. 2003 Mar;77(6):3851–3858. doi: 10.1128/JVI.77.6.3851-3858.2003

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FIG. 5. — Cell tropism of chimeric and pseudotyped virus MVMp and hH1 vectors. Several human (A) and mouse (B) cell lines were infected with recombinant standard, chimeric, or pseudotyped virus stocks at a MOI of 3 or 10 RU/cell, respectively. GFP (or EGFP) expression was monitored with a fluorescence microscope 48 and 72 h postinfection. The line with the highest number of fluorescent cells after infection with the indicated virus stock was set at 100% for GFP-positive cells. GFP-positive cells from the same cell line infected with all the other virus stocks were expressed relative to this reference value (given as a percentage). A minimum of 200 GFP-positive cells were counted in each cell line. Results are of one representative experiment (of three). (A) Relative number of GFP-positive human cells (U937 , U373 , and HeLa ) after infection with the indicated recombinant virus stocks. (B) Relative numbers of GFP-positive mouse cells (A9 , L929 , Ehrlich ascitic fluid , and C3H10T1/2 ) after infection with the indicated recombinant virus stocks.

Inline graphic — Cell tropism of chimeric and pseudotyped virus MVMp and hH1 vectors. Several human (A) and mouse (B) cell lines were infected with recombinant standard, chimeric, or pseudotyped virus stocks at a MOI of 3 or 10 RU/cell, respectively. GFP (or EGFP) expression was monitored with a fluorescence microscope 48 and 72 h postinfection. The line with the highest number of fluorescent cells after infection with the indicated virus stock was set at 100% for GFP-positive cells. GFP-positive cells from the same cell line infected with all the other virus stocks were expressed relative to this reference value (given as a percentage). A minimum of 200 GFP-positive cells were counted in each cell line. Results are of one representative experiment (of three). (A) Relative number of GFP-positive human cells (U937 , U373 , and HeLa ) after infection with the indicated recombinant virus stocks. (B) Relative numbers of GFP-positive mouse cells (A9 , L929 , Ehrlich ascitic fluid , and C3H10T1/2 ) after infection with the indicated recombinant virus stocks.