Skip to main content
. 2003 Mar;77(6):3516–3530. doi: 10.1128/JVI.77.6.3516-3530.2003

FIG. 3.

FIG. 3.

Construction of cell lines and populations that stably express the 2.0-kb LAT intron. (A) Schematic depiction of transgene expression cassettes introduced into cells by transfection prior to selection. Abbreviations: hCMVp, major IE promoter of human cytomegalovirus; IRES, internal ribosome entry site from encephalomyocarditis virus; BleoR, bleomycin resistance gene. (B) Northern blot hybridization analysis of control 293T cells (cont.), 293T cells 18 h after infection with HSV-1 strain KOS, and cell populations produced with the expression cassettes shown in panel A (293T-LAT). The blot was sequentially hybridized to probes for LAT and 18S rRNA. The 17 cell population expresses the 2.0-kb LAT intron at levels approximating those seen after wild-type infection at an MOI of 10. B, Bleo.