TABLE 1.
Immunohistologic study of the CNS during scrapie infection
| Mouse strain and cells | Expression in the CNS at wk postinoculationa
|
|||
|---|---|---|---|---|
| 6 | 10 | 12 | 18 | |
| C57BL/6 PrP+/+ | ||||
| CD8+ T | 0/4 | 0/4 | 4/4 | 4/4 |
| CD4+ T | 0/4 | 0/4 | 4/4 | 4/4 |
| MHC class I | 0/4 | 1/4 | 4/4 | 4/4 |
| MHC class II | 0/4 | 0/4 | 2/4 | 3/4 |
| GFAP+ | 4/4 | 4/4 | 4/4 | 4/4 |
| F4/80+ | 0/4 | 0/4 | 4/4 | 4/4 |
| Apoptosisb | 0/4 | 0/4 | 1/4 | 4/4 |
| MIP-1, IP-10 | 0/4 | ND | ND | 4/4 |
| RANTESc, C10c | 0/4 | ND | ND | 4/4 |
| MIP-2c | 1/4 | ND | ND | 4/4 |
| MCP-3, TCA-3c | 0/4 | ND | ND | 0/4 |
| BALB PrP+/+ | ||||
| CD8+ T | 0/4 | ND | 4/4 | 4/4 |
| CD4+ T | 0/4 | ND | 4/4 | 4/4 |
| MHC class I | 0/4 | ND | 4/4 | 4/4 |
| MHC class II | 0/4 | ND | 3/4 | 3/4 |
| GFAP+ | 4/4 | ND | 4/4 | 4/4 |
| F4/80+ | 0/4 | ND | 4/4 | 4/4 |
| Apoptosisb | 0/4 | ND | 4/4 | 4/4 |
| C57BL/6 PrP−/− | ||||
| CD8+ T | ND | ND | 0/3 | 0/3 |
| CD4+ T | ND | ND | 0/3 | 0/3 |
| MHC class I | ND | ND | 0/3 | 0/3 |
| Apoptosisb | ND | ND | 0/3 | 0/3 |
a
Expression of T cells in brains of mice and humans with TSE disease. Results are given as number of positive mice/total number of mice. ND, not determined
b
Apoptosis of neurons.
c
Chemokines in brains. Spleens were negative for chemokines at 6 and 18 weeks. Analysis by RPA.