FIG. 1.
Immunoblot analysis of treated HIV-1-infected cultures. (A) Time course immunoblots of virion samples produced from cells in the presence or absence of a 10 μM zLLL-lactacystin (zLLL/LC) combination. The number of hours posttreatment is indicated above each lane. (B) Immunoblot of virion samples produced from cells during 8 h of treatment with the indicated inhibitors. (C) Ubiquitin (Ub) immunoblots of cytoplasmic extracts from untreated and treated cells. The number of hours posttreatment is indicated above each lane. Ubn, polyubiquitinated proteins. (D) Ubiquitin immunoblots of nuclear extracts from untreated and treated cells. The number of hours posttreatment is indicated above each lane. The antiserum used is indicated above each blot, and each sample is labeled above its respective lane. “HIV-1” indicates virus control, a sample of a 24-h harvest of virions prior to treatment. Molecular mass markers are indicated on the left, and the detected bands are identified on the right.