FIG. 2.
Immunoblot analysis of treated MuLV-infected cultures. (A) Time course immunoblots of virion samples produced from cells in the presence or absence of 10 μM zLLL-lactacystin (zLLL/LC) combination. The number of hours posttreatment is indicated above each lane. (B) Ubiquitin immunoblots of cytoplasmic extracts from untreated and treated cells. The number of hours posttreatment is indicated above each lane. Ubn, polyubiquitinated proteins. (C) Ubiquitin immunoblots of nuclear extracts from untreated and treated cells. The number of hours posttreatment is indicated above each lane. (D) Immunoblot of virion samples after 8 h of treatment with the indicated inhibitors. The antiserum used is indicated above each blot, and each sample is labeled above its respective lane. “MuLV” indicates virus control, a sample of a 24-h harvest of virions prior to treatment. Molecular mass markers are indicated by arrows on the left, and the bands are identified by arrows on the right.