FIG. 1.

Interaction of Ad5-GFP and Ad5/35L-GFP vectors with HeLa cells. (A) Attachment and internalization of [³H]-thymidine-labeled Ad5-GFP and Ad5/35L-GFP vectors into HeLa cells. (B) Southern blot analysis of Ad genomes after attachment (Att) and internalization (Int) into HeLa cells. (C) Infection of HeLa cells with Ad vectors in the presence of different fiber knob domains. HeLa cells (2.5 × 10⁵ per well of a 12-well plate) were infected in triplicates with Ad5-GFP or Ad5/35LGFP vectors at indicated MOIs (virus particles per cell) in the absence (Control) or in the presence of a 100-μg/ml concentration of purified Ad5 or Ad35 knob domain. Twenty four hours later the efficiency of gene transfer was analyzed by flow cytometry.