Figure 2.

Syntaxin 17 is highly expressed in testosterone-synthesizing Leydig cells. Sections of rat testis were labeled with antibodies directed against syntaxin 17 (A and D) and VAMP4 (B and E). (C and F) Overlays of images in A and B and in D and E, respectively. Note that anti-syntaxin 17 antibodies strongly labeled the steroid hormone–secreting Leydig cells (Lc), whereas syntaxin 17 label was drastically reduced in cells of the seminiferous tubules (st) and in the lumen (lu) of the tubules. Bars, 400 μm for A, B, and C (in A) and 100 μm for D, E, and F (in D).