Figure 3.

Syntaxin 17 is abundantly expressed in steroid hormone–synthesizing cells of the adrenal cortex. (A and B) Sections of rat adrenal gland were double labeled with antibodies directed against syntaxin 17 (Texas Red–conjugated) and VAMP4 (FITC-conjugated). Syntaxin 17 is highly expressed in steroid hormone–synthesizing cells of the adrenal cortex (cx), whereas syntaxin 17 label is drastically reduced in the catecholamine-secreting cells of the adrenal medulla (md). Bars, 400 μm in A and 100 μm in B. (C) Bovine adrenal gland was dissected into cortex and medulla, postnuclear supernatant was prepared, and the expression of different SNARE proteins was analyzed by Western blotting. Labels on the right indicate the antibodies used for immunoblotting.