Figure 4.

Cell lines derived from Leydig cell and adrenal cortical cell tumors express high levels of syntaxin 17. Postnuclear supernatants (30 μg/lane) derived from the cell lines indicated on the top of the panel were separated by SDS-PAGE and immunoblotted with affinity-purified goat anti-syntaxin 17 antibodies. LC540 and R2C are cell lines derived from rat Leydig cell tumor, and H295R and Y-1 are cell lines derived from human and mouse adrenal tumors, respectively. The antibodies recognize a major band of 38 kDa in cell lines of rat or human origin and an ∼40-kDa band in the cell line of mouse origin. Note that only cell lines derived from steroidogenic tissues express high levels of syntaxin 17.