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. 2000 Aug;11(8):2719–2731. doi: 10.1091/mbc.11.8.2719

Figure 5.

Figure 5

Syntaxin 17 does not colocalize with markers of lysosomes, mitochondria, or RER. H295R adrenal cortical cells were fixed with 4% paraformaldehyde, permeabilized with saponin, and stained with affinity-purified goat anti-syntaxin 17 antibodies (A, D, and G). The cells were costained with mouse mAbs against LAMP1 (B) and cytochrome C (E) and rabbit antibodies against calnexin (H). Texas Red–labeled anti-goat IgG and FITC-labeled anti-mouse and anti-rabbit IgG were used as secondary antibodies. Cells were visualized with confocal microscopy. C, F, and I show merged images. Bars, 2 μm.