Syntaxin 17 does not colocalize with markers of
lysosomes, mitochondria, or RER. H295R adrenal cortical cells were
fixed with 4% paraformaldehyde, permeabilized with saponin, and
stained with affinity-purified goat anti-syntaxin 17 antibodies (A, D,
and G). The cells were costained with mouse mAbs against LAMP1 (B) and
cytochrome C (E) and rabbit antibodies against calnexin (H). Texas
Red–labeled anti-goat IgG and FITC-labeled anti-mouse and anti-rabbit
IgG were used as secondary antibodies. Cells were visualized with
confocal microscopy. C, F, and I show merged images. Bars, 2 μm.