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Movie 6. Analysis of nuclear GFP dynamics as a function of the cell division cycle. In Movie 6, the circled nucleus enters into prometaphase, at which point nucleoplasmic GFP disperses into the cytoplasm. Subsequent cell division is followed by reestablishment of an intact nuclear membrane within the daughter cells. GFP fluorescence then accumulates within the nuclei. Bar, 15 μm. The movie was created from 90 images collected at 1-min intervals.
