Table 1.

Effects of drugs on the movement of the root nuclei and nucleoplasmic GFP accumulation

Drug	Concentration (μM)	Time (h)	Response
Taxol	10	1	NEa
	10	6	NE
	20	6	NE
	20	24	NE
Oryzalin	10	1	NE
	10	4	NE
	20	4	NE
	20	16	NE
	50	4	NE
	100	4	GFP lost from the nucleoplasm
Vinblastine	5	3	NE
	5	24	NE
	20	1	NE
	100	1	NE
	500	1	Slight decrease in the velocity of some of the nuclei
Colchicine	10,000	65	NE
	50,000	4	NE
Latrunculin B	10	5 min	Movement stopped 15 min after completion of the treatment
	10	15 min	Movement stopped
Cytochalasin D	50	1	NE
	50	4	NE
	50	16	Velocity decreased
	100	4	NE
	100	16	Movement stopped in >90% of the nuclei
Cytochalasin B	50	1	NE
	50	5	NE
	100	4	Movement stopped
N-Ethylmaleimide	50	1	Movement stopped in the root hairs; some movement in the root body
	100	1	Movement stopped
	10,000	2	GFP lost from the nucleoplasm

^a NE, no effect. Nuclei continued movement, and GFP was retained within the nucleoplasm.