FIG. 3.

CD4⁺ and CD8⁺ murine T cells expressing hSLAM on their surfaces can be infected by both a wt MV after low passage in B95-8 marmoset cells (MV-JW_B) and a laboratory (MV-Ed) strain of MV. (A) Infection of splenic T lymphocytes with MV-JW_B. Activated CD4⁺ (graphs a and b) and CD8⁺ (graphs c and d) cells were mock infected (graphs a and c) or infected (graphs b and d) with MV-JW_B. Two days postinfection, MV proteins were detected by flow cytometry for tg (open histogram) and non-tg (filled histogram) mice. (B) Infection of T lymphocytes by the MV-Ed laboratory strain. Activated CD4⁺ (graphs a and b) and CD8⁺ (graphs c and d) lymphocytes were mock infected (graphs a and c) or infected (graphs b and d) with MV-Ed strain. Three days postinfection, cells were analyzed. An area of MV-positive cells was arbitrarily selected and compared to uninfected cells. The percentage of infected cells from non-tg mice was subtracted from the percentage of infected cells from tg mice; the results are shown in graphs b and d.