FIG. 3.

Transmembrane glycoprotein expression by engineered viruses. Vero cells infected with each of the engineered viruses and virus A2 were fixed with methanol at 16 h (for HRSV G) or 24 h (for proteins G^vsv and F) postinfection and incubated with antibody L9 directed against the HRSV G protein (αG), antibody MAb 19 directed against the HRSV F protein (αF), or an anti-VSIV G antibody (αG^vsv). After incubation with a secondary antibody carrying an Alexa-594 fluorescent conjugate, samples were examined on a fluorescence microscope and photographed (magnification, ×400). Uninfected Vero cells were included as a control (left panels).