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. 2000 Aug;11(8):2743–2756. doi: 10.1091/mbc.11.8.2743

Figure 5.

Figure 5

An APM-1 hybrid protein can complement UNC-101 proteins in vulval development: The apm-1 hybrid gene can rescue the suppression of vulvaless phenotype of unc-101(sy108). The animals in the photographs are at the L3 molt stage, which corresponds to the period of vulval induction. The arrowheads indicate the locations of the anchor cell that induces the vulval precursor cells (VPCs). The lines indicate the lineage of each VPC. The genotypes are: (A) wild-type; (B) let-23(sy1); (C) unc-101(sy108); let-23(sy1); (D) unc-101(sy108); let-23(sy1)::unc-101 minigene; (E) unc-101(sy108);let-23(sy1)::apm-1 hybrid gene; and (F) unc-101(sy108);let-23(sy1)::apm-2 hybrid gene. (A), wild-type vulval induction in which three vulval precursor cells (VPCs) have divided twice to make four progeny each. (B) There is no vulval induction because of the reduction-of-function mutation in the let-23EGFR gene. All VPCs have divided only once. (C) The unc-101 mutation suppresses the let-23(sy1) vulvaless mutant phenotype to greater-than-wild-type vulval induction. Four VPCs, instead of three VPCs as in normal induction, have divided twice. (D) The transgenic unc-101 minigene rescued the endogenous unc-101 (sy108) mutation, and thus no vulval induction occurred, as in the single mutant of let-23(sy1). (E) The apm-1 hybrid gene complemented the endogenous unc-101 (sy108) mutation, thus no vulval induction occurred, as in the single mutant of let-23(sy1). (F) Four VPCs were induced because the apm-2 hybrid gene could not complement the endogenous unc-101 (sy108) mutation. This phenotype is identical to that of unc-101(sy108); let-23(sy1) mutant animals. The scale bar is 10 μm.