Figure 6.

Redundancy of unc-101 and apm-1 in vulval development. The arrows indicate normal invagination of vulval tissues at the L4 stage in all figures. (A) An unc-101(sy108) animal showing wild-type vulval induction as indicated by a single invagination of the vulval tissue composed of the progeny of three VPCs. (B) An unc-101(sy108) animal injected with apm-1 double-strand RNA. An additional invagination by vulval cells, due to greater-than-wild-type induction, is indicated by an arrowhead. (C) Alet-23(sy1) animal injected with apm-1 double strand RNA. As in (B) an extra invagination is shown, as indicated by an arrowhead. (D) A transgenic animal of wild-type genetic background containing the apm-1::GFP reporter construct. An extra invagination is shown next to the normal vulval invagination. The scale bar is 10 μm.