Table 1.
Larval lethality caused by RNAi of apm-1 in N2 background
| High concentration
| |||
|---|---|---|---|
| Time after injection (h) | Eggs collected (n) | Arrested larvae (n) | Larval lethality (%) |
| 0–12 | 60 | 54 | 90 |
| 12–24 | 363 | 363 | 100 |
| 24–36 | 340 | 340 | 100 |
| 36–48 | 117 | 117 | 100 |
| 48–60 | 76 | 76 | 100 |
| Low concentration | |||
| Time after injection (h) | Eggs collected (n) | Arrested larvae (n) | Larval lethality (%) |
| 0–12 | 86 | 43 | 50 |
| 12–24 | 254 | 196 | 77 |
| 24–36 | 408 | 92 | 23 |
| 36–48 | 268 | 37 | 14 |
| 48–60 | 170 | 12 | 8 |
apm-1 double-strand RNA was injected into the gonads of N2 animals at either high (200 μg/ml) or low (20 μg/ml) concentration, and the arrested larvae were counted. Animals that did not arrest developed into adults without any significant defects. Note that there was no embryonic lethality.