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. 2003 Mar;77(6):3647–3654. doi: 10.1128/JVI.77.6.3647-3654.2003

FIG. 5.

FIG. 5.

FIG. 5.

F activation mediated by wild-type (wt) HN compared with that mediated by C28a HN, C28 HN, and P111S HN. The experimental strategy is described in the text. Cells expressing the indicated HNs and transfected with F were allowed to adsorb RBC at 4°C. They were then transferred to 22°C, and 4-GU-DANA was added either before (0 min) or 10, 20, or 60 min after transfer to 22°C. (A) The amount of RBC that remained bound 15 min after the addition of 4-GU-DANA was determined. These amounts, expressed as a percentage of total bound RBC (ordinate), are shown as a function of time at 22°C prior to the addition of 4-GU-DANA (abscissa). (B) Photographs were taken under a fluorescence microscope of the R18-labeled RBC on cell monolayers at the same time points as those shown in panel A.